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| 1 | Thomas Brown (Team Mountain Khakis p/b SmartStop) | 1:13:36 |
| 2 | Cesar Grajales (Realcyclist.com Pro Cycling Team) | 0:00:01 |
| 3 | Luke Keough (Team Mountain Khakis p/b SmartStop) | 0:00:12 |
| 4 | Frank Travieso (Realcyclist.com Pro Cycling Team) | Row 3 - Cell 2 |
| 5 | David Guttenplan (Chemstar p/b UnitedHealthcare) | Row 4 - Cell 2 |
| 6 | Demis Aleman (Jamis-Sutter Home) | Row 5 - Cell 2 |
| 7 | Emile Abraham (AeroCat Cycling Team) | 0:00:13 |
| 8 | Serghei Tvetcov (AeroCat Cycling Team) | Row 7 - Cell 2 |
| 9 | William Munoz (Deportivo CICO Rionegro) | Row 8 - Cell 2 |
| 10 | Patrick Weddell (Cleveland Clinic Sport) | Row 9 - Cell 2 |
| 11 | Daniel Holt (Team Type 1 Development) | 0:00:14 |
| 12 | Oscar Clark (Realcyclist.com Pro Cycling Team) | Row 11 - Cell 2 |
| 13 | Benjamin Zawacki (Team Mountain Khakis p/b SmartStop) | Row 12 - Cell 2 |
| 14 | Brendan Cornett (Locos Grill & Pub) | Row 13 - Cell 2 |
| 15 | Alexey Shmidt (Team Type 1-Sanofi) | Row 14 - Cell 2 |
| 16 | Gavriel Epstein (Chipotle Development Team) | 0:00:15 |
| 17 | Guido Palma (Jamis-Sutter Home) | 0:00:16 |
| 18 | Brian Toone (Tria Market p/b DonohooAuto.com) | 0:00:17 |
| 19 | Julian Cabra (Metro Reprographics) | Row 18 - Cell 2 |
| 20 | Nick Fishbein (Hincapie-Green Creation) | Row 19 - Cell 2 |
| 21 | Winston David (Globalbike p/b Catoma) | 0:00:18 |
| 22 | Christopher Monteleone (Kenda/5-hour Energy Pro Cycling p/b Geargrinder) | 0:00:19 |
| 23 | Rich Harper (Cleveland Clinic - RGF Cycling) | Row 22 - Cell 2 |
| 24 | Ricky Gargiulo (Colavita Racing) | Row 23 - Cell 2 |
| 25 | Travis Turner (Depaula Racing) | 0:00:20 |
| 26 | Stradford Helms (Team Type 1 Development) | Row 25 - Cell 2 |
| 27 | Andy Baker (Bissell Pro Cycling) | 0:00:21 |
| 28 | Neil Bezdek (Team Mountain Khakis p/b SmartStop) | 0:00:22 |
| 29 | Nick Housley (Chemstar p/b UnitedHealthcare) | 0:00:23 |
| 30 | Dylan Degan (Depaula Racing) | Row 29 - Cell 2 |
| 31 | Yosmani Pol (Team Coco's) | Row 30 - Cell 2 |
| 32 | Giancarlo Bianchi (WS United) | 0:00:25 |
| 33 | Andy Scarano (Chemstar p/b UnitedHealthcare) | 0:00:26 |
| 34 | Diego Garavito (AeroCat Cycling Team) | Row 33 - Cell 2 |
| 35 | Michael Midlarsky (Realcyclist.com Pro Cycling Team) | 0:00:28 |
| 36 | Justin Lowe (Hincapie Devo) | Row 35 - Cell 2 |
| 37 | Patrick Barnett (Carolina Velo-Cycle Center) | 0:00:30 |
| 38 | Mark Brown (Audi) | 0:00:32 |
| 39 | Brooks Bostick (Audi) | 0:00:33 |
| 40 | Leonardo Martinez (Team Coco's) | Row 39 - Cell 2 |
| 41 | Isaac Enderline (Hincapie Devo) | 0:00:37 |
| 42 | Mark Warno (AVC-Team Hagerstown) | 0:00:39 |
| 43 | Travis Livermon (Team Mountain Khakis p/b SmartStop) | 0:00:47 |
| 44 | John Delong (Hincapie-Green Creation) | 0:04:58 |
| 45 | Juan Arias (AeroCat Cycling Team) | Row 44 - Cell 2 |
| 46 | Joe Eldridge (Team Type 1-Sanofi) | Row 45 - Cell 2 |
| 47 | Sebastian Cancio (AeroCat Cycling Team) | 0:10:24 |
| 48 | Jerome Townsend (Team Mountain Khakis p/b SmartStop) | Row 47 - Cell 2 |
| 49 | Shane Braley (Chemstar p/b UnitedHealthcare) | Row 48 - Cell 2 |
| 50 | Caleb Welborn (Lone Star Racing Club) | Row 49 - Cell 2 |
| 51 | Philip Gaimon (Kenda/5-hour Energy Pro Cycling p/b Geargrinder) | Row 50 - Cell 2 |
| 52 | Jose Navarro (Team Coco's) | Row 51 - Cell 2 |
| 53 | Peter Kay (Globalbike p/b Catoma) | Row 52 - Cell 2 |
| 54 | AJ Meyer (Hincapie-Green Creation) | Row 53 - Cell 2 |
| 55 | Spencer Gaddy (Kenda/5-hour Energy Pro Cycling p/b Geargrinder) | Row 54 - Cell 2 |
| 56 | Simon Bennett (Globalbike p/b Catoma) | Row 55 - Cell 2 |
| 57 | Richard Kirby (Pre-Reg.com) | Row 56 - Cell 2 |
| 58 | David Lee (Metro Reprographics) | Row 57 - Cell 2 |
| 59 | Andrew Raab | Row 58 - Cell 2 |
| 60 | Andrea Ciacchinni (Team Type 1 Development) | Row 59 - Cell 2 |
| 61 | Bryan Schoeffler (Team OSP) | Row 60 - Cell 2 |
| 62 | Leonardo Marquez (AeroCat Cycling Team) | Row 61 - Cell 2 |
| 63 | Emilio Asconeguy (AeroCat Cycling Team) | Row 62 - Cell 2 |
| 64 | Damien Dunn (The Hub) | Row 63 - Cell 2 |
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| 1 | Sarah Fader | 0:50:14 |
| 2 | Lenore Pipes (MVP Health Care) | 0:00:01 |
| 3 | Addy Albershardt (MS-NOW) | 0:00:02 |
| 4 | Betty Hodges (Team Wooly Mammoth) | 0:00:03 |
| 5 | Jamie Dinkins (Motor Mile Racing) | Row 4 - Cell 2 |
| 6 | Zoe Frazier (Frazier Cycling) | Row 5 - Cell 2 |
| 7 | Hannah Coughlin (Team Kenda p/b Geargrinder) | Row 6 - Cell 2 |
| 8 | Peggy Legrand (US Military) | Row 7 - Cell 2 |
| 9 | Marianne Holt (Pain Pathways Women's Cycling) | Row 8 - Cell 2 |
| 10 | Nancy Jones (Subaru-Fisher Road Team) | 0:00:04 |
| 11 | Colle Paine (Team Kenda p/b Geargrinder) | 0:00:05 |
| 12 | Lauren De Crescenzo (Peachtree Bikes) | 0:01:25 |
| 13 | Mika Lyman (Boise Cycling Club) | Row 12 - Cell 2 |
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