| 1 | Meredith Miller (USA) California Giant/Specialized | 0:41:25 |
| 2 | Teal Stetson-Lee (USA) California Giant/Specialized | 0:00:01 |
| 3 | Susan Butler (USA) Hudz Subaru | 0:00:18 |
| 4 | Maureen Bruno Roy (USA) Bob's Red Mill pb Seven Cycles | 0:00:30 |
| 5 | Pepper Harlton (Can) Juventus Cycling Club | 0:00:37 |
| 6 | Coryn Rivera (USA) Marian University | 0:00:41 |
| 7 | Amanda Carey (USA) Kenda/Felt | 0:01:10 |
| 8 | Julie Krasniak (Fra) Team Rapha-Focus | 0:01:40 |
| 9 | Samantha Schneider (USA) CyclocrossRacing.com | 0:02:11 |
| 10 | Kelsy Bingham (USA) Roosters/Bikers Edge | 0:02:14 |
| 11 | Rebecca Blatt (USA) Rebecca Blatt | 0:02:49 |
| 12 | Brittany Mcconnell (USA) mercy specialized | 0:02:58 |
| 13 | Arley Kemmerer (USA) C3 - Athletes Serving Athletes | 0:03:07 |
| 14 | Serena Bishop (USA) Silverado Jewelry Gallery p/b Sunnyside Sports | 0:03:33 |
| 15 | Marne Smiley (USA) Bobs Red Mill | 0:03:56 |
| 16 | Corrie Osborne (USA) ISCorp | 0:04:17 |
| 17 | Corey Coogan Cisek (USA) Team Plan C p/b Stevens | 0:04:27 |
| 18 | Robin Williams (USA) Mercy-Specialized | 0:04:29 |
| 19 | Catherine Walberg (USA) Team Kenda p/b geargrinder | 0:04:37 |
| 20 | Elizabeth So (USA) Team TATI | 0:04:40 |
| 21 | Sarah Huang (USA) Nova IS Corp | 0:05:01 |
| 22 | Jeanne Fleck (USA) VELO Duluth-Twin Ports Cyclery | 0:05:28 |
| 23 | Kelly Paterson (USA) WOLVERINE/ACFSTORES.COM | 0:05:58 |
| 24 | Amber Rydholm (USA) New Hope 360/Trek/GS Boulder | Row 23 - Cell 2 |
