| 1 | Arley Kemmerer (USA) C3-Athletes Serving Athletes | 0:41:57 |
| 2 | Nicole Thiemann (USA) Team CF | Row 1 - Cell 2 |
| 3 | Erin Silliman (USA) Corsa Concepts Cyclocross Team | 0:00:29 |
| 4 | Cassandra Maximenko (USA) Silverbull targettraining | 0:00:36 |
| 5 | Brittlee Bowman (USA) pedalpowertraining.com | 0:00:44 |
| 6 | Katrina Dowidchuk (USA) Colavita ESPN W | Row 5 - Cell 2 |
| 7 | Patricia Buerkle (USA) Edlow Int p/b Bike Beat | 0:01:34 |
| 8 | Kathleen Wulfkuhle (USA) C3-Athletes Serving Athletes | 0:01:49 |
| 9 | Alice Henriques (USA) Zanconato Racing | 0:02:02 |
| 10 | Allyson Tufano (USA) Sportif Coaching Group/BPB | 0:02:10 |
| 11 | Stacey Barbossa (USA) Colavita/espnW NJ | 0:02:22 |
| 12 | Jessica Kutz (USA) Team EPS/CSS p/b Shebell & Shebell | 0:03:07 |
| 13 | Michelle Bishop (USA) North Point Women’s Racing | 0:03:20 |
| 14 | Bailey Semian (USA) Pure Energy Cycling-ProAirHFA | 0:03:44 |
| 15 | Tara Parsons (USA) CRCA | 0:04:28 |
| 16 | Elizabeth So (USA) Les Petites Victoires | 0:04:49 |
| 17 | Lauri Webber (USA) Wooden Wheels Racing | 0:05:11 |
| 18 | Amy Breyla (USA) C3-Athletes Serving Athletes | 0:05:40 |
| 19 | Erica Yozell Miller (USA) South Mountain Cycles | 0:06:26 |
| 20 | Diedre Ribbens (USA) C3-Athletes Serving Athletes | Row 19 - Cell 2 |
| 21 | Kristine Church (USA) Peanut Butter & Co. Human Zoom | 0:06:43 |
| 22 | Caitlin Thompson (USA) Peanut Butter & Co. Human Zoom | Row 21 - Cell 2 |
