140 riders take on the 2009 Holland Ladies Tour.
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| 1 | Charlotte Becker (Ger) Equipe Nürnberger Versicherung |
| 2 | Christina Becker (Ger) Equipe Nürnberger Versicherung |
| 3 | Suzanne De Goede (Ned) Equipe Nürnberger Versicherung |
| 4 | Marlen Johrend (Ger) Equipe Nürnberger Versicherung |
| 5 | Eva Lutz (Ger) Equipe Nürnberger Versicherung |
| 6 | Trixi Worrack (Ger) Equipe Nürnberger Versicherung |
| 11 | Linda Villumsen (Den) Team Columbia - HTC Women |
| 12 | Katherine Bates (Aus) Team Columbia - HTC Women |
| 13 | Chantal Beltman (Ned) Team Columbia - HTC Women |
| 14 | Ellen Van Dijk (Ned) Team Columbia - HTC Women |
| 15 | Emilia Fahlin (Swe) Team Columbia - HTC Women |
| 16 | Ina Teutenberg (Ger) Team Columbia - HTC Women |
| 21 | Janneke Kanis (Ned) DSB Bank - Nederland Bloeit |
| 22 | Adrie Visser (Ned) DSB Bank - Nederland Bloeit |
| 23 | Annemiek Van Vleuten (Ned) DSB Bank - Nederland Bloeit |
| 24 | Liesbeth De Vocht (Bel) DSB Bank - Nederland Bloeit |
| 25 | Marianne Vos (Ned) DSB Bank - Nederland Bloeit |
| 26 | Marieke Van Wanroij (Ned) DSB Bank - Nederland Bloeit |
| 31 | Emilie Aubry (Swi) Cervelo TestTeam |
| 32 | Lieselot Decroix (Bel) Cervelo TestTeam |
| 33 | Sarah Düster (Ger) Cervelo TestTeam |
| 34 | Pascale Schnider (Swi) Cervelo TestTeam |
| 35 | Patricia Schwager (Swi) Cervelo TestTeam |
| 36 | Kirsten Wild (Ned) Cervelo TestTeam |
| 41 | Saskia Elemans (Ned) Team Flexpoint |
| 42 | Mirjam Melchers-Van Poppel (Ned) Team Flexpoint |
| 43 | Loes Gunnewijk (Ned) Team Flexpoint |
| 44 | Loes Markerink (Ned) Team Flexpoint |
| 45 | Trine Schmidt (Den) Team Flexpoint |
| 46 | Iris Slappendel (Ned) Team Flexpoint |
| 51 | Emma Johansson (Swe) Red Sun Cycling Team |
| 52 | Elise Van Hage (Ned) Red Sun Cycling Team |
| 53 | Mascha Pijnenborg (Ned) Red Sun Cycling Team |
| 54 | Anne Arnouts (Bel) Red Sun Cycling Team |
| 55 | Inge Klep (Ned) Red Sun Cycling Team |
| 56 | Petra Dijkman (Ned) Red Sun Cycling Team |
| 61 | Andrea Bosman (Ned) Leontien.nl |
| 62 | Chantal Blaak (Ned) Leontien.nl |
| 63 | Irene Van Den Broek (Ned) Leontien.nl |
| 64 | Marlijn Binnendijk (Ned) Leontien.nl |
| 65 | Monique Van De Ree (Ned) Leontien.nl |
| 66 | Lucinda Brand (Ned) Leontien.nl |
| 71 | Sharon Van Essen (Ned) Team Ton van Bemmelen Sports / Merida |
| 72 | Roxane Kneteman (Ned) Team Ton van Bemmelen Sports / Merida |
| 73 | Hannah Welter (Ned) Team Ton van Bemmelen Sports / Merida |
| 74 | Judith Helmink (Ned) Team Ton van Bemmelen Sports / Merida |
| 75 | Anne Eversdijk (Ned) Team Ton van Bemmelen Sports / Merida |
| 76 | Amy Pieters (Ned) Team Ton van Bemmelen Sports / Merida |
| 81 | Nathalie Van Gogh (Ned) Swabo Ladies Cycling Team |
| 82 | Krista Meesterburrie (Ned) Swabo Ladies Cycling Team |
| 83 | Nina Kessler (Ned) Swabo Ladies Cycling Team |
| 84 | Aafke Eshuis (Ned) Swabo Ladies Cycling Team |
| 85 | Irene Tesink (Ned) Swabo Ladies Cycling Team |
| 86 | Sofie Van Horik (Ned) Swabo Ladies Cycling Team |
| 91 | Marissa Otten (Ned) Mixteam Merida Ladies/Rabo Lady Force |
| 92 | Sonja Boogaard (Ned) Mixteam Merida Ladies/Rabo Lady Force |
| 93 | Madelene Olssen (Swe) Mixteam Merida Ladies/Rabo Lady Force |
| 94 | Bianca Van Den Hoek (Ned) Mixteam Merida Ladies/Rabo Lady Force |
| 95 | Alike Haanstra (Ned) Mixteam Merida Ladies/Rabo Lady Force |
| 96 | Esmee Janssen (Ned) Mixteam Merida Ladies/Rabo Lady Force |
| 101 | Veronique Duivelshof (Ned) Mixteam TWC Het Snelle Wiel /Lotto-Belisol Ladies Team |
| 102 | Linda Schepers (Ned) Mixteam TWC Het Snelle Wiel /Lotto-Belisol Ladies Team |
| 103 | Imke Hartogs (Ned) Mixteam TWC Het Snelle Wiel /Lotto-Belisol Ladies Team |
| 104 | Kim Wouters (Ned) Mixteam TWC Het Snelle Wiel /Lotto-Belisol Ladies Team |
| 105 | Vera Koedooder (Ned) Mixteam TWC Het Snelle Wiel /Lotto-Belisol Ladies Team |
| 106 | Annemarieke Bruinsma (Ned) Mixteam TWC Het Snelle Wiel /Lotto-Belisol Ladies Team |
| 111 | Hielke Elferink (Ned) Cycling Team Tubanters |
| 112 | Pippa Handley (GBr) Cycling Team Tubanters |
| 113 | Berdienke Paus (Ned) Cycling Team Tubanters |
| 114 | Nathalie Joling (Ned) Cycling Team Tubanters |
| 115 | Moniek Tenniglo (Ned) Cycling Team Tubanters |
| 116 | Annika Heijnen (Ned) Cycling Team Tubanters |
| 121 | Judith Bloem (Ned) GRC Jan van Arckel/Nutrixxion |
| 122 | Claudia Meyer (Ger) GRC Jan van Arckel/Nutrixxion |
| 123 | Petra Van Wijgerden (Ned) GRC Jan van Arckel/Nutrixxion |
| 124 | Wilma Korevaar (Ned) GRC Jan van Arckel/Nutrixxion |
| 125 | Olivia Koster (Ned) GRC Jan van Arckel/Nutrixxion |
| 126 | Marieke Den Otter (Ned) GRC Jan van Arckel/Nutrixxion |
| 131 | Mariëlle Kerste (Ned) Restore Cycling Ladies Team |
| 132 | Hilde Van Slochteren (Ned) Restore Cycling Ladies Team |
| 133 | Eva Heijmans (Ned) Restore Cycling Ladies Team |
| 134 | Eshter Kortekaas (Ned) Restore Cycling Ladies Team |
| 135 | Mirjam Van Dijk (Ned) Restore Cycling Ladies Team |
| 141 | Winanda Spoor (Ned) Mixteam Zuidwesthoek/WV Schijndel/Spartaan |
| 142 | Jorinda De Heer (Ned) Mixteam Zuidwesthoek/WV Schijndel/Spartaan |
| 143 | Sofie Verdonck (Bel) Mixteam Zuidwesthoek/WV Schijndel/Spartaan |
| 144 | Suzan Simons (Ned) Mixteam Zuidwesthoek/WV Schijndel/Spartaan |
| 145 | Susanne Van As (Ned) Mixteam Zuidwesthoek/WV Schijndel/Spartaan |
| 146 | Amanda Bongaards (Ned) Mixteam Zuidwesthoek/WV Schijndel/Spartaan |
| 151 | Martine Bras (Ned) Selle Italia Ghezzi |
| 153 | Giada Borgato (Ita) Selle Italia Ghezzi |
| 154 | Agne Bagdonaviciute (Ltu) Selle Italia Ghezzi |
| 155 | Gloria Presti (Ita) Selle Italia Ghezzi |
| 156 | Laura Bozzolo (Ita) Selle Italia Ghezzi |
| 161 | Silke Kogelman (Ned) Profile Peoples Trust Cycling Team |
| 162 | Judith Jelsma (Ned) Profile Peoples Trust Cycling Team |
| 163 | Marieke Van Nek (Ned) Profile Peoples Trust Cycling Team |
| 164 | Sanne Fictoor (Ned) Profile Peoples Trust Cycling Team |
| 165 | Judith Visser (Ned) Profile Peoples Trust Cycling Team |
| 166 | Joukje Braam (Ned) Profile Peoples Trust Cycling Team |
| 171 | Jolien D'hoore (Bel) Topsport Vlaanderen Thompson Ladies Team |
| 172 | Kelly Druyts (Bel) Topsport Vlaanderen Thompson Ladies Team |
| 173 | Jessie Daams (Bel) Topsport Vlaanderen Thompson Ladies Team |
| 174 | Sjoukje Dufoer (Bel) Topsport Vlaanderen Thompson Ladies Team |
| 175 | Ine Beyen (Bel) Topsport Vlaanderen Thompson Ladies Team |
| 176 | Loes Sels (Bel) Topsport Vlaanderen Thompson Ladies Team |
| 181 | Anne Heijkoop (Ned) Batavus Ladies Cycling Team |
| 182 | Joan Boskamp (Ned) Batavus Ladies Cycling Team |
| 183 | Anne De Wildt (Ned) Batavus Ladies Cycling Team |
| 184 | Erika Broekema (Ned) Batavus Ladies Cycling Team |
| 185 | Adriënne Snijder (Ned) Batavus Ladies Cycling Team |
| 186 | Julia Soek (Ned) Batavus Ladies Cycling Team |
| 191 | Danielle Bekkering (Ned) Team Hitec Products Uck |
| 192 | Eyelien Bekkering (Ned) Team Hitec Products Uck |
| 193 | Janneke Ensing (Ned) Team Hitec Products Uck |
| 194 | Margriet Kloppenburg (Den) Team Hitec Products Uck |
| 195 | Sara Mustonen (Swe) Team Hitec Products Uck |
| 196 | Isabelle Söderberg (Swe) Team Hitec Products Uck |
| 201 | Emma Trott (GBr) Mixteam Weijers-Hako-Moving Ladies |
| 202 | Kate Cullen (GBr) Mixteam Weijers-Hako-Moving Ladies |
| 203 | Chloe Hosking (Aus) Mixteam Weijers-Hako-Moving Ladies |
| 204 | Rosyida Dahlina (Ina) Mixteam Weijers-Hako-Moving Ladies |
| 205 | Maria Luisa Calle Williams (Col) Mixteam Weijers-Hako-Moving Ladies |
| 206 | Elisabeth Aqudelo (Col) Mixteam Weijers-Hako-Moving Ladies |
| 211 | Kimberly Van De Berg (Ned) Mixteam Noordwesthoek/ Pedaalridders |
| 212 | Carolin Gustafsson (Swe) Mixteam Noordwesthoek/ Pedaalridders |
| 213 | Samantha Van Steenis (Ned) Mixteam Noordwesthoek/ Pedaalridders |
| 214 | Elke Riedl (Aut) Mixteam Noordwesthoek/ Pedaalridders |
| 215 | Pelin Cizgin (Aut) Mixteam Noordwesthoek/ Pedaalridders |
| 216 | E Mollema (Ned) Mixteam Noordwesthoek/ Pedaalridders |
| 221 | Alexandra Greenfield (GBr) British National Team |
| 222 | Sarah Reynolds (GBr) British National Team |
| 223 | Corrine Hall (GBr) British National Team |
| 224 | Jessica Booth (GBr) British National Team |
| 225 | Ella Sadler Andrews (GBr) British National Team |
| 231 | Sigrid Kuizenga (Ned) Mixteam Hepro Kozijnen/ NWV Groningen |
| 232 | Lisanne Ottema (Ned) Mixteam Hepro Kozijnen/ NWV Groningen |
| 233 | Emma Crum (NZl) Mixteam Hepro Kozijnen/ NWV Groningen |
| 234 | Gerda Koops (Ned) Mixteam Hepro Kozijnen/ NWV Groningen |
| 235 | Rachel Neylan (Aus) Mixteam Hepro Kozijnen/ NWV Groningen |
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