| 1 | Marianne Vos (Ned) Nederland Bloeit | 2:32:03 |
| 2 | Judith Arndt (Ger) HTC Highroad Women | 0:00:42 |
| 3 | Emma Johansson (Swe) Hitec Products - UCK | Row 2 - Cell 2 |
| 4 | Annemiek Van Vleuten (Ned) Nederland Bloeit | Row 3 - Cell 2 |
| 5 | Pauline Ferrand Prevot (Fra) France | Row 4 - Cell 2 |
| 6 | Noemi Cantele (Ita) Garmin - Cervelo | Row 5 - Cell 2 |
| 7 | Liesbet De Vocht (Bel) Topsport Vlaanderen 2012 - Ridley Team | 0:01:06 |
| 8 | Rochelle Gilmore (Aus) Lotto Honda Team | Row 7 - Cell 2 |
| 9 | Christine Majerus (Lux) GSD | Row 8 - Cell 2 |
| 10 | Lucinda Brand (Ned) AA Drink - Leontien.nl Cycling Team | Row 9 - Cell 2 |
| 11 | Lise Nöstvold (Nor) Hitec Products - UCK | Row 10 - Cell 2 |
| 12 | Charlotte Becker (Ger) HTC Highroad Women | Row 11 - Cell 2 |
| 13 | Grace Verbeke (Bel) Topsport Vlaanderen 2012 - Ridley Team | Row 12 - Cell 2 |
| 14 | Vicki Whitelaw (Aus) Lotto Honda Team | Row 13 - Cell 2 |
| 15 | Sara Mustonen (Swe) Hitec Products - UCK | Row 14 - Cell 2 |
| 16 | Elodie Hegoburu (Fra) France | Row 15 - Cell 2 |
| 17 | Oxana Kozonchuk (Rus) Diadora - Pasta Zara | Row 16 - Cell 2 |
| 18 | Janneke Ensing (Ned) Dolmans Landscaping Team | Row 17 - Cell 2 |
| 19 | Sharon Laws (GBr) Garmin - Cervelo | Row 18 - Cell 2 |
| 20 | Fröydis Waerstad (Nor) Hitec Products - UCK | Row 19 - Cell 2 |
| 21 | Loes Gunnewijk (Ned) Nederland Bloeit | Row 20 - Cell 2 |
| 22 | Evelyn Stevens (USA) HTC Highroad Women | Row 21 - Cell 2 |
| 23 | Sofie De Vuyst (Bel) Lotto Honda Team | Row 22 - Cell 2 |
| 24 | Maaike Polspoel (Bel) Topsport Vlaanderen 2012 - Ridley Team | Row 23 - Cell 2 |
| 25 | Pascale Jeuland (Fra) Vienne Futuroscope | Row 24 - Cell 2 |
| 26 | Jessie Daams (Bel) Garmin - Cervelo | Row 25 - Cell 2 |
| 27 | Marion Rousse (Fra) Vienne Futuroscope | Row 26 - Cell 2 |
| 28 | Dani King (GBr) | Row 27 - Cell 2 |
| 29 | Marie Lindberg (Swe) Abus Nutrixxion | Row 28 - Cell 2 |
| 30 | Nathalie Lamborelle (Lux) Abus Nutrixxion | Row 29 - Cell 2 |
| 31 | Desiree Schuler (Ger) Germany | Row 30 - Cell 2 |
| 32 | Amanda Spratt (Aus) Australia | Row 31 - Cell 2 |
| 33 | Irene Van Den Broek (Ned) AA Drink - Leontien.nl Cycling Team | Row 32 - Cell 2 |
| 34 | Pascale Schnider (Swi) | Row 33 - Cell 2 |
| 35 | Ashleigh Moolman (RSA) Lotto Honda Team | Row 34 - Cell 2 |
| 36 | Edwige Pitel (Fra) Vienne Futuroscope | Row 35 - Cell 2 |
| 37 | Elena Berlato (Ita) Top Girls Fassa Bortolo | Row 36 - Cell 2 |
| 38 | Mélodie Lesueur (Fra) France | Row 37 - Cell 2 |
| 39 | Linda Melanie Villumsen (NZl) AA Drink - Leontien.nl Cycling Team | Row 38 - Cell 2 |
| 40 | Tiffany Cromwell (Aus) Lotto Honda Team | Row 39 - Cell 2 |
| 41 | Laure Werner (Bel) Lotto Honda Team | Row 40 - Cell 2 |
| 42 | Patricia Schwager (Swi) Nederland Bloeit | Row 41 - Cell 2 |
| 43 | Simona Frapporti (Ita) Top Girls Fassa Bortolo | Row 42 - Cell 2 |
| 44 | Julie Beveridge (Can) Vienne Futuroscope | 0:01:14 |
| 45 | Emilia Fahlin (Swe) HTC Highroad Women | Row 44 - Cell 2 |
| 46 | Shara Gillow (Aus) Australia | 0:01:16 |
| 47 | Nicole Cooke (GBr) SC MCipollini Giordana | Row 46 - Cell 2 |
| 48 | Amber Neben (USA) HTC Highroad Women | Row 47 - Cell 2 |
| 49 | Trixi Worrack (Ger) AA Drink - Leontien.nl Cycling Team | Row 48 - Cell 2 |
| 50 | Alona Andruk (Ukr) Diadora - Pasta Zara | Row 49 - Cell 2 |
| 51 | Sanne Bamelis (Bel) | 0:01:19 |
| 52 | Rossella Callovi (Ita) SC MCipollini Giordana | Row 51 - Cell 2 |
| 53 | Iris Slappendel (Ned) Garmin - Cervelo | 0:01:27 |
| 54 | Tatiana Guderzo (Ita) SC MCipollini Giordana | Row 53 - Cell 2 |
| 55 | Leah Guloien (Can) | 0:01:30 |
| 56 | Inga Cilvinaite (Ltu) Diadora - Pasta Zara | Row 55 - Cell 2 |
| 57 | Alexandra Carle (Aus) Australia | 0:01:32 |
| 58 | Mélanie Bravard (Fra) GSD | 0:01:47 |
| 59 | Marta Bastianelli (Ita) SC MCipollini Giordana | Row 58 - Cell 2 |
| 60 | Emma Silversides (GBr) | 0:02:05 |
| 61 | Eneritz Iturriagaechevarria Mazaga (Spa) Lointek | 0:02:29 |
| 62 | Inge Klep (Ned) | Row 61 - Cell 2 |
| 63 | Karol-Ann Canuel (Can) Vienne Futuroscope | Row 62 - Cell 2 |
| 64 | Anne Arnouts (Bel) | Row 63 - Cell 2 |
| 65 | Gloria Presti (Ita) Top Girls Fassa Bortolo | 0:02:46 |
| 66 | Claire Thomas (GBr) | 0:02:54 |
| 67 | Alna Burato (Fra) France | 0:03:50 |
