| 1 | Kendall Ryan (Team TIBCO/To the Top) | 1:01:21 |
| 2 | Jennifer Purcell (Team TIBCO/To the Top) | 0:01:08 |
| 3 | Erica Allar (RideClean / PatentIt.com) | Row 2 - Cell 2 |
| 4 | Sarah Fader (Pepper Palace/Spin-Tech Training p/b ABRC) | 0:01:09 |
| 5 | Joanne Kiesanowski (Team TIBCO/To the Top) | Row 4 - Cell 2 |
| 6 | Laura Van Gilder (Mellow Mushroom/Rose Bandit Racing) | Row 5 - Cell 2 |
| 7 | Alexis Ryan (Team TIBCO/To the Top) | Row 6 - Cell 2 |
| 8 | Lindsay Bayer (XO Communications p/b Cisco) | Row 7 - Cell 2 |
| 9 | Debbie Milne (Absolute Racing/ MSMOC) | 0:01:10 |
| 10 | Addyson 'Addy' Albershardt (NOW-Novartis for MS) | Row 9 - Cell 2 |
| 11 | Kat Carr (Paceline Projects p/b Veloforma) | Row 10 - Cell 2 |
| 12 | Carrie Cash (Pedal the Cause) | Row 11 - Cell 2 |
| 13 | Elizabeth Morse Hill (Z Motion Hill) | 0:01:11 |
| 14 | Sara Clafferty (ABRT) | 0:01:12 |
| 15 | Morgan Patton (Team Type 1-Sanofi) | 0:01:13 |
| 16 | Christy Keely (Team Kenda presented by Geargrinder) | 0:01:16 |
| 17 | Samantha Schneider (Team TIBCO/To the Top) | 0:01:17 |
| 18 | Erin Burton (Cleveland Clinic Sports Health) | 0:01:18 |
| 19 | Christine Roettger (Pedal the Cause) | 0:01:19 |
| 20 | Melissa Ross (Team Kenda presented by Geargrinder) | Row 19 - Cell 2 |
| 21 | Marianne Holt (PainPathways Cycling Team) | 0:01:20 |
| 22 | Miller Hannah (PainPathways Cycling Team) | 0:01:21 |
| 23 | Valeria Galeano (Mellow Mushroom/Rose Bandit Racing) | 0:01:22 |
| 24 | Cheryl Fuller Muller (Louis Garneau Factory Team) | Row 23 - Cell 2 |
| 25 | Lauren Komanski (PainPathways Cycling Team) | 0:01:24 |
| 26 | Vanessa Drigo (Vanderkitten-Focus) | 0:01:26 |
| 27 | Kristen Meshberg (Spidermonkey) | Row 26 - Cell 2 |
| 28 | Kathryn Clark (Team Kenda presented by Geargrinder) | 0:01:33 |
| 29 | Arley Kemmerer (MVP Health Care Cycling) | 0:01:36 |
| DNF | Jennie May (Louis Garneau Factory Team) | Row 29 - Cell 2 |
| DNF | Alice Wallet (MVP Health Care Cycling) | Row 30 - Cell 2 |
| DNF | Amy Phillips (Mystique Cycling Team / SCV) | Row 31 - Cell 2 |
| DNF | Kate Ross (Pepper Palace/Spin-Tech Training p/b ABRC) | Row 32 - Cell 2 |
| DNF | Setter Jocelyn (Stranamanti Cycling) | Row 33 - Cell 2 |
| DNF | Colleen Paine (Team Kenda presented by Geargrinder) | Row 34 - Cell 2 |
| DNF | Rebecca Schepps (Team Type 1-Sanofi) | Row 35 - Cell 2 |
| DNF | Mandy Marquardt (Team Type 1-Sanofi) | Row 36 - Cell 2 |
| DNF | Prouty Debbie | Row 37 - Cell 2 |
