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| 1 | Katerina Nash (Luna Pro Team) | 0:42:00 |
| 2 | Pavla Havlíková (AVB Cycling Team) | 0:00:46 |
| 3 | Jana Kyptová (Johnson Controls Aš Mb) | 0:03:20 |
| 4 | Zuzana Pirzkallová (KC Kooperativa Sg Jablonec N.N) | 0:05:24 |
| 5 | Karolína Kalašová (Scott Cycling Team Kolín) | 0:06:13 |
| 6 | Jitka Škarnitzlová (Dimp - Giant Team) | 0:06:50 |
| 7 | Pavlína Marácková (S.K. Jir(Í Team Ostrava) | 0:09:31 |
| 8 | Romana Danecková (ACS Drak Vrbno) | -1lap |
| 9 | Michaela Ištvánová (ACS Stará Ves N. Ondr Ejnicí) | Row 8 - Cell 2 |
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| 1 | Zdenek Štybar (Telenet Fidea Cycling Team) | 1:00:04 |
| 2 | Petr Dlask (Telenet Fidea Cycling Team) | 0:00:38 |
| 3 | Martin Bína (Cyklo Team Budvar Tábor) | 0:00:47 |
| 4 | Martin Zlámalík (Volvo Auto Hase Mtb Team) | Row 3 - Cell 2 |
| 5 | Radomír Ml. Šimunek (BKCP - Powerplus) | 0:02:24 |
| 6 | Ondrej Bambula (Cyklo Team Budvar Tábor) | 0:02:40 |
| 7 | Lubomír Petruš (BKXP - Powerplus) | 0:03:20 |
| 8 | Zdenek Mlynár (Max Cursor) | 0:03:24 |
| 9 | Jirí Polnický (Sunweb - Projob Cycling Team) | 0:03:43 |
| 10 | Kamil Ausbuher (Prodoli Remerx Team) | 0:03:48 |
| 11 | Jan Škarnitzl (Dimp - Giant Team) | Row 10 - Cell 2 |
| 12 | Jan Nesvadba (KC Kooperativa Sg Jablonec N.N) | 0:03:56 |
| 13 | Vladimír Kyzivát (Johnson Controls Aš Mb) | 0:04:36 |
| 14 | Karel Hník (Telenet Fidea Cycling Team) | 0:04:56 |
| 15 | Filip Eberl (Scott Cycling Team Kolín) | 0:05:36 |
| 16 | David Menger (Prodoli Remerx Team) | 0:05:48 |
| 17 | Petr Novotný (TJ Stadion Louny) | 0:06:02 |
| 18 | Tomáš Janošek (ACK Stará Ves N. Ondr Ejnicí) | 0:06:49 |
| 19 | Michal Benda (Johnson Controls Aš Mb) | 0:07:32 |
| 20 | Filip Adel (Cyklo Team Budvar Tábor) | 0:08:09 |
| 21 | Josef Soukup (Duratec) | 0:08:46 |
| 22 | Michal Šimerle (KC Kooperativa Sg Jablonec N.N) | 0:08:57 |
| 23 | Bretislav Rohel (Forman Cycling Team) | 0:09:08 |
| 24 | Ivo Plevák (Max Cursor) | 0:09:10 |
| 25 | Karel Nepraš (Ack Stará Ves N. Ondr Ejnicí) | 0:09:42 |
| 26 | Tomáš Podrazil (TJ Sokol Holé Vrchy) | 0:09:50 |
| 27 | Martin Rehák (Scott Cycling Team Kolín) | -1lap |
| 28 | Jakub Rohlík (Cyklo Team Budvar Tábor) | -2laps |
| 29 | Jakub Friml (Scott Cycling Team Kolín) | Row 28 - Cell 2 |
| 30 | Petr Linhart (TJ Sokol Holé Vrchy) | Row 29 - Cell 2 |
| 31 | Lukáš Kovanda (Cyklo Team Budvar Tábor) | Row 30 - Cell 2 |
| 32 | Pavel Jelínek (Kc Slavia Praha) | Row 31 - Cell 2 |
| 33 | Josef Suchý (Johnson Controls Aš Mb) | -3laps |
| 34 | Marek Farkaš (TJ Lokomotiva Beroun) | -4laps |
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