| 1 | Katerina Nash (Cze) Luna Pro Team | 0:38:46 |
| 2 | Meredith Miller (USA) Cal Giant Berry Farms/Specialized | 0:00:15 |
| 3 | Caroline Mani (Fra) Raleigh-Clement | 0:00:16 |
| 4 | Pepper Harlton (Can) Juventus Cycling Club | 0:00:18 |
| 5 | Nicole Duke (USA) Raleigh-Clement | 0:00:27 |
| 6 | Julie Krasniak (Fra) Rapha-Focus | 0:01:02 |
| 7 | Courtenay McFadden (USA) Clif Bar//Bicycle Centres/Voler | 0:01:20 |
| 8 | Mical Dyck (Can) Stan's NoTubes Elite Cyclocross Team | 0:01:58 |
| 9 | Alice Pennington (USA) Kona | 0:02:01 |
| 10 | Nicole Thiemann (USA) Team CF | 0:02:04 |
| 11 | Andrea Smith (USA) LadiesFirst Racing | 0:02:31 |
| 12 | Serena Gordon (USA) Silverado p/b Sunnyside Sports | 0:02:41 |
| 13 | Anna Dingman (USA) Real Wheels Bicycle Studio | 0:03:35 |
| 14 | Rebecca Blatt (USA) Van Dessel | 0:03:39 |
| 15 | Alexis Ryan (USA) The Team SoCalCross | 0:03:50 |
| 16 | Emily Kachorek (USA) | 0:04:02 |
| 17 | Katie Melena (USA) Bicycle Blue Book/HRS/Rock Lobster Cycles | 0:04:09 |
| 18 | Ellen Sherrill (USA) Bicycle Bluebook/HRS/Rock Lobster | 0:04:15 |
| 19 | Beth Ann Orton (USA) Team S&M | 0:05:14 |
| 20 | Hannah Finchamp (USA) Cynergy Cycles Racing Team | 0:05:32 |
| 21 | Selene Yeager (USA) | 0:05:53 |
| 22 | Shannon Gibson (USA) Stan's NoTubes Elite Cyclocross Team | 0:07:14 |
| 23 | Maddie Melcher (USA) | 0:07:59 |
