| 1 | Judith Arndt (Ger) HTC Highroad Women | 0:45:10 |
| 2 | Amber Neben (USA) HTC Highroad Women | 0:00:20 |
| 3 | Wendy Houvenaghel (GBr) Great Britain | 0:00:26 |
| 4 | Olga Zabelinskaya (Rus) Russian Federation | 0:00:40 |
| 5 | Emma Pooley (GBr) Garmin - Cervelo | 0:00:43 |
| 6 | Tara Whitten (Can) TIBCO - To The Top | 0:00:48 |
| 7 | Shara Gillow (Aus) Bizkaia-Durango | 0:00:55 |
| 8 | Julia Shaw (GBr) Great Britain | 0:01:04 |
| 9 | Evelyn Stevens (USA) HTC Highroad Women | 0:01:22 |
| 10 | Pascale Schnider (Swi) | 0:01:50 |
| 11 | Tatiana Antoshina (Rus) Gauss | 0:01:54 |
| 12 | Hanka Kupfernagel (Ger) | 0:01:58 |
| 13 | Pia Sundstedt (Fin) Finland | 0:02:21 |
| 14 | Vicki Whitelaw (Aus) Lotto Honda Team | 0:02:46 |
| 15 | Sharon Laws (GBr) Garmin - Cervelo | 0:03:02 |
| 16 | Taryn Heather (Aus) Australia | 0:03:35 |
| 17 | Svetlana Pauliukaite (Ltu) Colavita Forno d'Asolo | 0:03:42 |
| 18 | Tiffany Cromwell (Aus) Hitec Products - UCK | 0:03:52 |
| 19 | Thrude Karlsen (Nor) Norway | 0:04:08 |
| 20 | Tetyana Riabchenko (Ukr) Colavita Forno d'Asolo | 0:04:33 |
| 21 | Camilla Hott-Johansen (Nor) Norway | 0:04:59 |
| 22 | Carla Ryan (Aus) Garmin - Cervelo | 0:05:36 |
| 23 | Michelle Lauge Jensen (Den) Denmark | 0:06:31 |
| 24 | Lucie De Carlo (Fra) | 0:08:05 |
