| 1 | Wendy Houvenaghel (GBr) | 0:46:01 |
| 2 | Julia Shaw (GBr) | 0:01:06 |
| 3 | Grace Verbeke (Bel) Lotto-Belisol Ladiesteam | 0:01:17 |
| 4 | Iris Slappendel (Ned) Team Flexpoint | 0:01:20 |
| 5 | Bridie O'donnell (Aus) | 0:01:21 |
| 6 | Vera Koedooder (Ned) Lotto-Belisol Ladiesteam | 0:01:49 |
| 7 | Pascale Schnider (Swi) Cervelo TestTeam | 0:02:52 |
| 8 | Elizabeth Armitstead (GBr) Lotto-Belisol Ladiesteam | 0:03:09 |
| 9 | Shara Gillow (Aus) | 0:03:33 |
| 10 | Audrey Cordon (Fra) Vienne Futuroscope | 0:03:56 |
| 11 | Pippa Handley (GBr) | 0:04:15 |
| 12 | Annick Le Helley (Fra) | 0:04:55 |
| 13 | Lotte Van Hoek (Ned) | 0:05:19 |
| 14 | Debora Galvez Lopez (Spa) Lointek | 0:05:24 |
| 15 | Winanda Spoor (Ned) | 0:05:40 |
| 16 | Fiona Dutriaux (Fra) Vienne Futuroscope | 0:05:52 |
| 17 | Alna Burato (Fra) | 0:06:20 |
| 18 | Birgit Lavrijssen (Ned) | 0:06:36 |
| 19 | Jennifer Fischer (Fra) Vienne Futuroscope | 0:06:38 |
