| 1 | Amber Pierce (Diadora Pasta Zara) | 0:58:20 |
| 2 | Tiffany Pezzulo (Primal/MapMyRide) | 0:00:34 |
| 3 | Katerina Nash (Luna Pro Team) | Row 2 - Cell 2 |
| 4 | Emily Collins (Vanderkitten) | Row 3 - Cell 2 |
| 5 | Mary Elizabeth Maroon (Webcor/Alto Velo) | Row 4 - Cell 2 |
| 6 | Rebecca Werner (Webcor/Alto Velo) | Row 5 - Cell 2 |
| 7 | Jane Robertson (Metromint Cycling) | Row 6 - Cell 2 |
| 8 | Alison Powers (Team TIBCO/To The Top) | Row 7 - Cell 2 |
| 9 | Liza Rachetto (Primal/MapMyRide) | Row 8 - Cell 2 |
| 10 | Jennifer Weinbrecht (Vanderkitten-Focus) | Row 9 - Cell 2 |
| 11 | Alison Starnes (Peanut Butter & Co. TWENTY12) | Row 10 - Cell 2 |
| 12 | Elizabeth Newell (Fremont Bank Cycling Team) | Row 11 - Cell 2 |
| 13 | Dulce Pliego-Moreno (Ruse) | 0:00:40 |
| 14 | Emily Thurston (Touchstone Climbing & Fitness) | 0:00:44 |
| 15 | Carlee Taylor (Team TIBCO/To The Top) | 0:00:46 |
| 16 | Starla Teddergreen (VANDERKITTEN - FOCUS) | 0:02:19 |
| 17 | Heather Nielson (Touchstone Climbing) | Row 16 - Cell 2 |
| 18 | Martina Patella | Row 17 - Cell 2 |
| 19 | Jennifer Reither (Vanderkitten Focus) | Row 18 - Cell 2 |
| 20 | Nichole Wangsgard (Team Primal Racing) | Row 19 - Cell 2 |
| DNS | Alexis Ryan (Team TIBCO) | Row 20 - Cell 2 |
| DNS | Haley Juno-Galdes (Webcor/Alto Velo) | Row 21 - Cell 2 |
| DNS | Holly Liske (Webcor/Alto Velo) | Row 22 - Cell 2 |
| DNS | Jane Wolcott (Webcor/Alto Velo) | Row 23 - Cell 2 |
| DNS | Emily Matheu (Third Pillar) | Row 24 - Cell 2 |
| DNS | Courtney Dimpel (Vanderkitten) | Row 25 - Cell 2 |
| DNF | Kathryne Carr (Bell Lap Coaching) | Row 26 - Cell 2 |
| DNF | Rhonda Serr (Dolce Vita Cycling) | Row 27 - Cell 2 |
| DNF | Michelle Melka (Red Racing) | Row 28 - Cell 2 |
| DNF | Heather Lipana (Rio Strada Racing) | Row 29 - Cell 2 |
| DNF | Tammy Wildgoose (SC Velo - Empower Coaching) | Row 30 - Cell 2 |
| DNF | Tara McCormick (Team TIBCO) | Row 31 - Cell 2 |
| DNF | Marissa Axell (Touchstone Climbing) | Row 32 - Cell 2 |
| DNF | Emily Foxman (Webcor/Alto Velo) | Row 33 - Cell 2 |
| DNF | Jazzy Hurkino (Vanderkitten-Focus) | Row 34 - Cell 2 |
