| 1 | Summer Moak (Rally UHC Cycling) | 1:01:15 |
| 2 | Laura Jorgensen (Fast Chance Cycling) | Row 1 - Cell 2 |
| 3 | Daniely Garcia (Cwa Racing pb Trek) | Row 2 - Cell 2 |
| 4 | Lily Williams (Hagens Berman Superm) | 0:00:01 |
| 5 | Christina Gokey-smith (Colavita Bialetti Racing) | Row 4 - Cell 2 |
| 6 | Emily Ehrlich (Cwa Racing pb Trek) | Row 5 - Cell 2 |
| 7 | Whitney Allison (Hagens Berman Superm) | Row 6 - Cell 2 |
| 8 | Janelle Cole (Fast Chance Cycling) | 0:00:03 |
| 9 | Faith Montreuil (Gray Goat - Bullseye Total Med) | 0:00:05 |
| 10 | Harriet Owen (Hagens Berman Superm) | 0:00:26 |
| 11 | Jennifer Caicedo (Finish Strong) | Row 10 - Cell 2 |
| 12 | Tina Pic (Colavita Bialetti Racing) | Row 11 - Cell 2 |
| 13 | Natalia Franco (Colavita Bialetti Racing) | Row 12 - Cell 2 |
| 14 | Rachel Langdon (Fast Chance Cycling) | Row 13 - Cell 2 |
| 15 | Erica Carney (Rally UHC Cycling) | 0:00:27 |
| 16 | Starla Teddergreen (Hagens Berman Superm) | Row 15 - Cell 2 |
| 17 | Hannah Shell (Dna Pro Cycling Team) | Row 16 - Cell 2 |
| 18 | Debbie Milne (Supra Cycling Team) | 0:00:28 |
| 19 | Ana Casas (Cwa Racing pb Trek) | Row 18 - Cell 2 |
| 20 | Manuela Escobar (Milligan Cycling) | Row 19 - Cell 2 |
| 21 | Mia Kilburg | Row 20 - Cell 2 |
| 22 | Natalia Franco (ColavitaBialetti Pro Cycling) | 0:00:29 |
| 23 | Kat Sweatt (Specialized Wolfpack pb Jakroo) | 0:00:30 |
| 24 | Madeline Bemis (Milligan College) | Row 23 - Cell 2 |
| 25 | Madison Kelly (Cwa Racing pb Trek) | 0:00:31 |
| 26 | Merrill Thierman | Row 25 - Cell 2 |
| 27 | Regina Legge (Gray Goat - Bullseye Total Med) | 0:00:32 |
| 28 | Courteney Lowe (Fast Chance Womens Cycling) | 0:00:33 |
| 29 | Sommers Creed | Row 28 - Cell 2 |
| 30 | Jolene Holland-neve (Colavita Bialetti Racing) | Row 29 - Cell 2 |
| 31 | Julie Kuliecza (Hagens Berman Supermint) | 0:00:34 |
| 32 | Gracie Pendleton (Milligan College) | Row 31 - Cell 2 |
| 33 | Judah Sencenbaugh (Savannah College Of Art And Des) | 0:00:36 |
| 34 | Caroline Baur (Cwa Racing pb Trek) | 0:00:39 |
| 35 | Winada Spoor (Fast Chance Cycling) | 0:00:42 |
| 36 | Samantha Schneider (Cwa Racing pb Trek) | 0:00:49 |
