| 1 | Carmen Small (USA) Specialized-lululemon | 0:24:51.80 |
| 2 | Joelle Numainville (Can) Optum p/b Kelly Benefit Strategies | 0:00:28.00 |
| 3 | Chantal Blaak (Ned) Team TIBCO | 0:00:35.59 |
| 4 | Janel Holcomb (USA) Optum p/b Kelly Benefit Strategies | 0:00:39.29 |
| 5 | Lauren Hall (USA) Optum p/b Kelly Benefit Strategies | 0:00:45.72 |
| 6 | Claudia Häusler (Ger) Team TIBCO | 0:00:49.60 |
| 7 | Shelley Olds (USA) Team TIBCO | 0:00:58.13 |
| 8 | Jamie Bookwalter (USA) Colavita Fine Cooking | 0:01:03.15 |
| 9 | Denise Ramsden (Can) Team Canada | 0:01:05.87 |
| 10 | Lex Albrecht (Can) NOW and Novartis for MS | 0:01:14.12 |
| 11 | Stephanie Roorda (Can) GSD Gestion Kallisto | 0:01:15.88 |
| 12 | Alizee Brien (Can) GSD Gestion Kallisto | 0:01:19.76 |
| 13 | Catherine Dessureault (Can) Stevens Racing p/b The Cyclery | 0:01:22.27 |
| 14 | Laura Brown (Can) Colavita Fine Cooking | 0:01:27.52 |
| 15 | Katarzyna Pawlowska (Pol) GSD Gestion Kallisto | 0:01:37.67 |
| 16 | Melanie Späth (Irl) Team TIBCO | 0:01:56.90 |
| 17 | Paolina Allan (Can) P-K Expresss/HNZ Strategic | 0:01:57.56 |
| 18 | Laura Gazzola (Can) P-K Expresss/HNZ Strategic | 0:02:05.65 |
| 19 | Kinley Gibson (Can) Team Canada | 0:02:28.58 |
| 20 | Ellen Watters (Can) West Quebec Wheelers | 0:02:31.79 |
| 21 | Ally Stacher (USA) Specialized-lululemon | 0:02:34.88 |
| 22 | Adriane Provost (Can) Stevens Racing p/b The Cyclery | 0:02:37.39 |
| 23 | Annie Foreman-Mackey (Can) Stevens Racing p/b The Cyclery | 0:02:40.01 |
| 24 | Gabrielle Pilot-Fortin (Can) Équipe du Québec | 0:02:40.06 |
| 25 | Jamie Gilgen (Can) Infinit Canada Cycle | 0:02:53.98 |
| 26 | Melinda Spratt (USA) L.G. Factory | 0:03:06.77 |
| 27 | Julia Bradley (Can) MVP Health Care | 0:03:20.52 |
| 28 | Stephanie Skoreyko (Can) Infinit Canada Cycle | 0:03:24.55 |
| 29 | Janie Rioux Coulombe (Can) Équipe du Québec | 0:03:57.10 |
| 30 | Allyson Gillard (Can) Équipe du Québec | 0:04:05.76 |
| 31 | Natalie Koch (USA) MVP Health Care | 0:04:13.03 |
| 32 | Marie-Claude Molnar (Can) Canada Para National | 0:05:14.55 |
| 33 | Lindsay Aspen (Can) West Quebec Wheelers | 0:06:11.36 |
